Isolation of Mitochondria and Mitochondrial RNA from Crithidia fasciculata
نویسندگان
چکیده
منابع مشابه
Mitochondrial DNA ligase in Crithidia fasciculata.
Kinetoplast DNA (kDNA), the form of mitochondrial DNA in trypanosomatids, consists of thousands of interlocked circular DNAs organized into a compact disk structure. A type II DNA topoisomerase, a DNA polymerase beta, and a structure-specific endonuclease have been localized to antipodal sites flanking the kDNA disk along with nascent DNA minicircles. We have cloned a gene (LIG k) encoding a mi...
متن کاملIsolation and characterization of drug resistant mutants of Crithidia fasciculata.
Mutants of Crithidia fasciculata, resistant to Actinomycin D, Tubercidin, Crystal Violet, 6-Azauridine, and 5-Fluorouracil were obtained and characterized. The mutants were stable when maintained under nonselective conditions, and could be grouped in several subclasses on the basis of cross-resistance to other drugs. Actinomycin D- and Crystal Violet-resistant mutants appeared spontaneously whe...
متن کاملMidpoint potentials of the mitochondrial cytochromes of Crithidia fasciculata.
The midpoint potentials of the mitochondrial respiratory chain cytochromes of the protozoan Crithidia fasciculata at pH 7.2, Em7.2, show great similarity to those measured in higher organisms. Values of Em7.2 for cytochromes a and a3 are +165 and +340 mV. Both c cytochromes have Em7.2 = +230 mV. There are two b cytochromes with the same spectral characteristics with Em7.2 = -20 and -135 mV. The...
متن کاملIsolation and characterization of kinetoplast DNA networks and minicircles from Crithidia fasciculata.
7. Cook, J. R. 1968. The cultivation and growth of Euglena, in Buetow, D. E., ed., The Biology of Euglena, Academic Press, New York 1, 243-314. 8. Cooper, T. G. & Beevers, H. 1969. P-oxidation in glyoxysomes from castor bean endosperm. J . Biol. Chem. 244, 3514-20. 9. Cramer, M. & Myers, J. 1952. Growth and photosynthetic characteristics of Euglena gracilis. Arch. Mikrobiol. 17, 384-402. 10. Da...
متن کاملThe growth and nutrition of Crithidia fasciculata.
The conditions for luxuriant axenic cultivation of the trypanosomid flagellate Crithidia fasciculata in chemically-defined media were determined. Improvements over the hitherto published media include higher concentrations of amino acids, folic acid (where this is the sole source of pteridine) and purines and the inclusion of threonine and Tween 80. Increased oxygenation was obtained by incubat...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of General Microbiology
سال: 1977
ISSN: 0022-1287
DOI: 10.1099/00221287-99-2-291